Image of the Week - July 18, 2002

View full-sized.

Montage of 3D Animation of Confocal Microscope Point Spread Functions

Fluorescent 190 nanometer diameter polystyrene spheres imaged through laser scanning confocal microscopy (<a href="">animated version</a>). The image illustrates the distortion of axial (z-plane) resolution due to spherical aberration arising from an index mis-match of the mounting media. The spheres were imaged through 20 microns of glycerol-gelatin using a 63x 1.32NA oil immersion objective and 543 nanometer wavelength light. The field of view (each frame) is 740 nanometers.

Image Courtesy:


Uploaded Sun Jul 21 11:19:02 2002 by Karl Garsha

Updated Tue Jan 26 22:24:48 2010 by Daniel E. Weber