Image of the Week - March 7, 2006

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Standard Wide-Field Fluorescence Compared to Structured Illumination

In these images of bovine pulmonary artery endothelial cells, the mitochondria are stained with MitoTracker Red CMXRos (red), and F-actin is stained with BODIPY FL phallacidin (green). On the left is a standard wide-field fluorescence image that includes signal fluorescence from above and below focus. On the right is the same image following deconvolution using the Zeiss Apotome Structured Illumination System.

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Contact: ekman@itg.uiuc.edu

Uploaded Tue Mar 7 09:06:13 2006 by Jonathan Ekman

Updated Wed Feb 3 09:49:33 2010 by Daniel E. Weber