Equipment

Listed below are the major microscopes, ancillary equipment, and facilities available to users of the Microscopy Suite. Click the equipment name for more information on each piece of equipment.

Please be aware that the ITG staff cannot respond to questions about particular pieces of equipment or software packages unless those questions are related to research conducted in the ITG laboratories. The ITG does not sell, lease, or distribute equipment or software, save that clearly marked as developed by the ITG.


Inspection Light Microscope
Zeiss AxioScope A1, 100 W Hal lamp designed for UV-blocked epi-illumination, Zeiss Axiovision LE software facilitates image collection. Zeiss AxioCam ICc3 CCD digital camera.

Dissecting Light Microscope (Stereoscope)
Zeiss SV11 stereomicroscope, Hg lamp for low-magnification fluorescence, epi-fluorescent illumination, PixeLink digital camera.

Zeiss SteREO Discovery V20 Microscope
Zeiss Stereo Zoom Discovery V.20 Light Microscope with Vision Research Micro eX4 Monochrome High Speed Video Camera

Fluorescence Light Microscope
Fluorescence, phase contrast, high NA DIC. Zeiss Axiovision software facilitates image collection. B/W EMCCD & color CCD cameras, Zeiss Apotome structured illumination system, mark & find, time-lapse, z-stacking, stage heater, motorized stage & automated shutters to minimize light exposure.

Micro-Spectroscopy Workstation
Zeiss Axio Observer D1 inverted microscope for visible to near-InfraRed (NIR) spectroscopy (300-1700 nm). Cooled color CCD for basic imaging, InGaAs NIR camera to image thermal and optical emission in the NIR region and single point micro-Raman spectroscopy is available with 531 nm laser line.

Multiphoton Fluorescence Confocal Microscope (FCS/FLIM)
ISS Alba FCS instrument with single-photon (One 470 nm diode laser) and multiphoton (Ti:Sapphire laser) excitation, combined with a laser scanning module. It is capable of Fluorescence Correlation Spectroscopy (FCS), Fluorescence Lifetime Imaging Microscopy (FLIM) and Fluorescence Resonance Energy Transfer (FRET) imaging.

Fluorescence Resonance Energy Transfer (FRET) Microscope
The Zeiss Axiovert 200M is equipped with a high sensitivity cooled Photometrics 512 Evolve EMCCD camera. The system is configured to be capable of fluorescence resonance energy transfer (FRET) microscopy for a number of well-known FRET pairs, such as ECFP-YPet.

Stereology Workstation
StereoInvestigator and Neurolucida software. Trace and analyze branching structures, plot and analyze distribution of cells throughout single or serial sections. System includes all modern stereological probes for unbiased measurements. Zeiss AxioImager A1 light microscope, Optronics MicroFire Progressive Scan, motorized stage, stereological analysis tools.

Leica SP8 UV/Visible Laser Confocal Microscope
405, 458, 488, 514, 561 & 633 nm laser lines, 28 fps Full Range Resonance Scanning, Tiling, FRAP, FRET.

Environmental Scanning Electron Microscope
ESEM with field-emission electron gun, EDAX energy-dispersive spectroscopy (light-element capability), Peltier-effect heating/cooling stage, 1000° and 1500° C heating stages, Everhart-Thornley secondary electron detector (SED), three gaseous SEDs (GSEDs; for imaging in 'wet' mode), backscattered electron detectors, S-VHS recorder, electrical feedthroughs, other options.

Transmission Electron Microscope
Philips CM200 TEM operates at accelerating voltages of up to 200 kV. Digital image acquisition using a TVIPS 2k x 2k Peltier-cooled CCD camera. Compustage for precise control of specimen holder/specimen movement, low-dose kit, high tilt holder, rotation stage holder.

Atomic Force Microscope (MultiMode AFM)
Digital Instruments Nanoscope IIIa, capable of contact, tapping, magnetic force, electric force, phase imaging, and pulsed force modes; scanning tunnelling microscopy (STM) head; J-scanner with x,y range of 125 microns and vertical range of 5 microns; A-scanner with x,y range of 1 micron and vertical range of 400 nm; analyses may be performed in air or liquid cell.

Near-Field Scanning Optical Microscope
Apertured NSOM system built to run on modified AFM software. Facilitates (e.g.) surface plasmon resonance (SPR) imaging.

Raman Confocal Imaging System
The Raman confocal imaging microscope is a high-resolution research-grade Horiba LabRAM HR 3D-capable Raman spectroscopy imaging system that is optimized for the visible-to-NIR spectral range.

Xradia MicroCT
A high-resolution 3D X-ray imaging system with sub-1 micron pixel resolution for samples up to 100 mm in diameter and 1 kg weight.

Xradia Bio MicroCT
A high-resolution 3D X-ray imaging system for sample up to 200 mm in diameter and 15 kg weight.

Xradia NanoCT
An ultra high-resolution full volume imaging system capable of producing 3D images with resolution down to 50 nm.

Glow Discharge System
Glow discharge is a mild argon-plasma etch of the surface of carbon-stabilized Formvar-coated TEM grids that removes atmospheric contaminants on TEM grids, rendering them hydrophilic for several days.

Millipore Water Purifier
Takes water from the Beckman Institute’s large-scale reverse osmosis/mixed-bed/UV-sterilized water purification system and runs it through another set of filters/cartridges.

Low Speed Bone Saw
A precision water-cooled rotary saw intended primarily for fine sectioning of SEM samples.

AccuSizer FX Particle Sizer
Focused extinction system for the determination of complex multimodal particle size distributions between 0.7 and 20 microns.

Carbon Evaporator
High-vacuum turbo-pumped Denton evaporator with capacity for carbon thread, carbon rod, resistive metal evaporation, dual evaporation mode.

Critical Point Dryer
Enables nondestructive drying of biological, biomaterial, and material samples from 100% ethanol.

Dual Source Metal Evaporator
Used for high vacuum coating of carbon and other materials as an adjunct to TEM, SEM, x-ray microanalysis, and other research methodologies.

Fiber Puller
Laser controlled instrument for pulling glass fibers to very fine tips; used primarily for NSOM.

NICOMP 380 ZLS Particle Sizer
Dynamic light scattering instrument for determining the particle size distribution of particles in a liquid.

Sputter Coater
Turbo-pumped sputter coater with rotating/tilting stage, film thickness monitor; various targets, including Au, Au/Pd, Pt, Al, Ti.

Ultramicrotome
Multi-illumination of the pyramid and knife edge, dual automatic advance system, control unit with cutting speed control, memory for five combinations of advance/cutting speed settings, return speed and cutting window settings. Capable of ultrathin sectioning from 50 nm to maximum 5 micrometers.


Facilities

Wet Lab
Bench space, fume hood, some chemicals and solvents, dual source metal evaporator, pH meter, dissecting scope, microbalance, glow discharge system, reverse osmosis water purification system, sonicator, sputter coater, critical point dryer, low speed bone saw, osmium plasma coater, and other equipment.

Clean Room
A small Class 500 Clean Room laboratory available for specimen preparation. The Clean Room is for creation and inspection of MEMs devices such as microstamps or microfluidic devices. ITG users need to reserve time to use the Clean Room.