Fluorescence Light Microscope (LM)


The Zeiss Observer Z1 inverted research-grade microscope is configured to perform fluorescence microscopy as well as differential interference contrast (DIC or Nomarski) and phase contrast microscopy. The addition of a stage heater, a motorized stage, and a set of shutters to minimize light exposure allows live cell imaging. The entire system is driven using Zen, a sophisticated Zeiss software package which facilitates image collection and system control. The installed ZEN Tiles & Positions module allows scanning specimen in large area and aligning the individual images precisely in relation to one another, and provides the optimal basis for imaging and analyzing the area larger than the field of view. The microscope is equipped with three advanced cameras - two Zeiss cameras, the monochromatic AxioCam MRm and the color Axiocam 503 for normal image acquisition, and a Roper Scientific Cascade 512B EMCCD camera for high performance imaging in low light applications.

Tables for the Zeiss Objectives can be found here.
A chart for immersion oil types can be found here.

For additional information about this piece of equipment, see the Calendars, Contacts, and Fees pages.

Primary ContactsDianwen Zhang, Mark Bee
ManufacturerCarl Zeiss, Inc.
Equipment ModelAxiovert 200M
Features- High resolution color CCD
- B/W EMCCD with 90% QE
- High resolution color CCD
- Phase contrast high NA DIC
- Zeiss Axiovision Software
- Time-lapse
- Z-stacking
- Mark & Find
- Tiling
- Apotome structured illumination
- Stage heater
- Motorized stage
- Shutters to minimize light exposure
LocationB606B
Phone(217) 265-5071
(217) 244-0170
Related EquipmentClean Room
User ResourcesGeneral Information and Operating Procedures
AxioVision LE Rel. 4.7 viewer software
Filter sets