Fluorescence Light Microscope (LM)

The Zeiss Axiovert 200M inverted research-grade microscope is configured to perform fluorescence microscopy as well as differential-interference contrast (Nomarski) and phase-contrast microscopy. The addition of a stage heater, motorized stage, and shutters to minimize light exposure allows users of the facility to perform live cell microscopy. The entire system is driven using Zeiss Axiovision, a sophisticated software package which facilitates image collection. A particularly useful feature of this software is the ability to montage multiple fields of view, allowing composition of images of very large specimens. Images may be acquired using a Roper Scientific Cascade 512B EMCCD camera, a Zeiss AxioCam MRm camera or a Zeiss AxioCam HRC color CCD camera. The system is also equipped with Zeiss Apotome structured illumination system to increase widefield fluorescence contrast and for optical sectioning.

Tables for the Zeiss Objectives can be found here.
A chart for immersion oil types can be found here.

For additional information about this piece of equipment, see the Calendars, Contacts, and Fees pages.

Primary ContactsDianwen Zhang, Mark Bee
ManufacturerCarl Zeiss, Inc.
Equipment ModelAxiovert 200M
Features- High resolution color CCD
- B/W EMCCD with 90% QE
- High resolution color CCD
- Phase contrast high NA DIC
- Zeiss Axiovision Software
- Time-lapse
- Z-stacking
- Mark & Find
- Tiling
- Apotome structured illumination
- Stage heater
- Motorized stage
- Shutters to minimize light exposure
Phone(217) 265-5071
(217) 244-0170
Related EquipmentClean Room
User ResourcesGeneral Information and Operating Procedures
AxioVision LE Rel. 4.7 viewer software
Filter sets