Leica SP8 UV/Visible Laser Confocal Microscope


Confocal microscopy permits one to optically section a fluorescent sample (such as a cell that has been stained with contrasting fluorescent dyes) with superior resolution by using a pinhole to reject light that originates outside of the chosen area. By collecting a series of such images through the depth of a sample, the user may assemble a highly accurate three-dimensional reconstruction of the entire sample.

For additional information about this piece of equipment, see the Calendars, Contacts, and Fees pages.

Primary ContactDianwen Zhang
LocationB420B
Phone(217) 244-6292
ManufacturerLeica Microsystems
Equipment ModelTCS SP8
Objectives
  • 20x/0.75 IMM (oil, water, glycerol) CORR CS2, free Working distance 670 um
  • 40x/1.30 HC PL APO Oil CS2, free Working distance 240 um
  • 63x/1.40 HC PL APO Oil CS2, free working distance 140 um
Features
  • Laser lines: 405, 458, 488, 514, 561 & 633 nm.
  • Four detectors: Three PMTs + one high-sensitivity GaAsP HyD detectors.
  • Resonant scanning (8kHz) allows 28 fps at 512*512 for fast live cell imaging.
  • Scan optics for high transmission from 400 nm to 1300 nm.
  • Optical scanfield rotation up to 200 degrees.
  • Three mirrors X2Y scanner for parallax-free and tiling imaging.
  • Motorized stage, mark & find, tiling confocal imaging.
  • LAS X control software.
Application Modules
  • Fluorescence Recovery After Photobleaching (FRAP).
  • Fluorescence Resonance Energy Transfer (FRET).
User Resources