Leica SP8 UV/Visible Laser Confocal Microscope
Confocal microscopy permits one to optically section a fluorescent sample (such as a cell that has been stained with contrasting fluorescent dyes) with superior resolution by using a pinhole to reject light that originates outside of the chosen area. By collecting a series of such images through the depth of a sample, the user may assemble a highly accurate three-dimensional reconstruction of the entire sample.
For additional information about this piece of equipment, see the Calendars, Contacts, and Fees pages.
|Primary Contact||Dianwen Zhang|
|Equipment Model||TCS SP8|
- 20x/0.75 IMM (oil, water, glycerol) CORR CS2, free Working distance 670 um
- 40x/1.30 HC PL APO Oil CS2, free Working distance 240 um
- 63x/1.40 HC PL APO Oil CS2, free working distance 140 um
- Laser lines: 405, 458, 488, 514, 561 & 633 nm.
- Four detectors: Three PMTs + one high-sensitivity GaAsP HyD detectors.
- Resonant scanning (8kHz) allows 28 fps at 512*512 for fast live cell imaging.
- Scan optics for high transmission from 400 nm to 1300 nm.
- Optical scanfield rotation up to 200 degrees.
- Three mirrors X2Y scanner for parallax-free and tiling imaging.
- Motorized stage, mark & find, tiling confocal imaging.
- LAS X control software.
- Fluorescence Recovery After Photobleaching (FRAP).
- Fluorescence Resonance Energy Transfer (FRET).