This web site displays a series of light and confocal micrographs illustrating a variety of subcellular structures and organelles. We hope to provide a useful educational resource for people interested in cytology who do not have access to advanced imaging technologies or cell biological expertise.
We have employed a cultured epithelial cell line, CV1-monkey kidney cells, for these experiments and stained for such organelles as the Golgi apparatus, the endoplasmic reticulum, nucleus, mitochondria, and several cytoskeletal elements. Protocols for each staining as well as a description of the microscopes utilized are provided.
Confocal and fluorescent microscopy images help to reveal the intriguing structures of the cell. We hope also to demonstrate the high quality imaging techniques available for such visualization. This project has been made possible with the support of the Beckman Institute of Advanced Science and Technology's Imaging Technology Group at the University of Illinois at Champaign-Urbana.