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Montage of 3D Animation of Confocal Microscope Point Spread Functions

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Created 07/18/2002

Fluorescent 190 nanometer diameter polystyrene spheres imaged through laser scanning confocal microscopy (http://www.micromaestro.org animated version). The image illustrates the distortion of axial (z-plane) resolution due to spherical aberration arising from an index mis-match of the mounting media. The spheres were imaged through 20 microns of glycerol-gelatin using a 63x 1.32NA oil immersion objective and 543 nanometer wavelength light. The field of view (each frame) is 740 nanometers.

Credits

  • Karl Garsha , ITG, Beckman Institute
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