Microscopy Suite Equipment
Listed below are the major microscopes, ancillary equipment, and facilities available to users of the Microscopy Suite. Click the equipment name for more information on each piece of equipment.
Please be aware that the ITG staff cannot respond to questions about particular pieces of equipment or software packages unless those questions are related to research conducted in the ITG laboratories. The ITG does not sell, lease, or distribute equipment or software, save that clearly marked as developed by the ITG.
3D X-ray Computed TomographyCT Lab GX series, for ultra-high-speed, high-resolution 3D X-ray micro CT. Using the Sample-Stationary Method, these new devices can perform CT scans in 8 seconds at top speed, with a minimum resolution of 4.5 µm. Rigaku Nano3DX X-ray Microscope A high-resolution 3D X-ray imaging system for sample up to 200 mm in diameter and 15 kg weight. A high-resolution 3D X-ray imaging system with sub-1 micron pixel resolution for samples up to 100 mm in diameter and 1 kg weight.
3D X-ray Computed Tomography Prep EquipmentA precision water-cooled rotary saw intended primarily for fine sectioning of SEM samples.
Confocal ImagingThe Deep UV Raman confocal imaging microscope is a home-built spectral imaging system that is optimized for the UV-to-visible spectral range. 405, 458, 488, 514, 561 & 633 nm laser lines, 28 fps Full Range Resonance Scanning, Tiling, FRAP, FRET. ISS Alba FCS instrument with single-photon and multiphoton excitation, plus laser scanning module. Capable of FCS, FLIM and FRET imaging. The Raman confocal imaging microscope is a high-resolution research-grade Horiba LabRAM HR 3D-capable Raman spectroscopy imaging system that is optimized for the visible-to-NIR spectral range. The Bruker Ultima upright multiphoton microscope is capable of simultaneous or sequential imaging, electrophysiology and uncaging/photostimulation on in vivo and in vitro specimens.
Electron MicroscopyESEM with field-emission electron gun, EDAX energy-dispersive spectroscopy, Peltier-effect heating/cooling stage, 1000µ and 1500µ C heating stages, Everhart-Thornley SED, three GSEDs, backscattered electron detectors, electrical feedthroughs, and more. Philips CM200 TEM operates at accelerating voltages of up to 200 kV. Digital image acquisition using a TVIPS 2k x 2k Peltier-cooled CCD camera. Offers precise control of specimen, low-dose kit, high tilt holder, rotation stage holder.
Electron Microscopy Prep EquipmentEnables nondestructive drying of biological, biomaterial, and material samples from 100% ethanol. High-vacuum turbo-pumped Denton evaporator with capacity for carbon thread, carbon rod, resistive metal evaporation, dual evaporation mode. Turbo-pumped sputter coater with rotating/tilting stage, film thickness monitor; various targets, including Au, Au/Pd, Pt, Al, Ti. The Desk V Sample Preparation system, equipped for both evaporation and sputtering Diamond Knife (use with UCT if available) Used for high vacuum coating of carbon and other materials as an adjunct to TEM, SEM, X-ray microanalysis, and other research methodologies. Glow discharge is a mild argon-plasma etch of the surface of carbon-stabilized Formvar-coated TEM grids that removes atmospheric contaminants on TEM grids, rendering them hydrophilic for several days. High Pressure Freezing Hydrogen Plasma Cleaner Oxygen Plasma Asher Enables nondestructive drying of biological, biomaterial, and material samples from 100% ethanol. For the preparation of thin sections for electron microscopy Multi-illumination pyramid and knife edge, dual automatic advance system, control unit with cutting speed control, memory for five speed combination settings. Capable of ultrathin sectioning from 50 nm to maximum 5 micrometers.
Light Microscopy/SpectroscopyDeben Tensile/Compression Stage Fluorescence, phase contrast, high NA DIC. Software facilitated image collection. Zeiss AxioScope A1, 100 W Hal lamp designed for UV-blocked epi-illumination, Zeiss Axiovision LE software facilitates image collection. Zeiss AxioCam ICc3 CCD digital camera. Linkam Heating and Cooling Stage Inverted microscope for visible to near-infrared (NIR) spectroscopy (300-1700 nm) Remote Stereology Workstation Trace, plot, and analyze structures and cell distribution. System includes all modern stereological probes, Zeiss AxioImager A1 light microscope, Optronics MicroFire Progressive Scan, motorized stage, stereological analysis tools. The Zeiss Axiovert 200M is equipped with a high sensitivity cooled EMCCD camera. The system is capable of fluorescence resonance energy transfer (FRET) microscopy for a number of well-known FRET pairs, such as ECFP-YPet. Zeiss Stereo Zoom Discovery V.20 Light Microscope with Vision Research Micro eX4 Monochrome High Speed Video Camera.
Light Scattering Particle-Sizing SystemsFocused extinction system for the determination of complex multimodal particle size distributions between 0.7 and 20 microns. The Litesizer™ 500 is an instrument for characterizing nano- and microparticles in dispersions and solutions. The NS300 allows rapid, automated analysis of the size distribution and concentration of all types of nanoparticles from 10nm to 2000nm in diameter, depending on the instrument configuration and sample type. Dynamic light scattering instrument for determining the particle size distribution of particles in a liquid.
Other Preparation EquipmentLaser controlled instrument for pulling glass fibers to very fine tips; used primarily for NSOM.
Scanned Probe Microscopy and ProfilometryCapable of contact, tapping, phase imaging, and multiple force modes in air or liquid cell; STM head; J-scanner with x,y range of 125 microns and vertical range of 5 microns; A-scanner with x,y range of 1 micron and vertical range of 400 nm. The P-6 stylus profiler continues the tradition of best-in-class technology and performance by incorporating many features from KLA-Tencor's automated P-16+ stylus profiler.
Wet Lab - B612
Bench space, fume hood, some chemicals and solvents, dual source metal evaporator, pH meter, dissecting scope, microbalance, glow discharge system, reverse osmosis water purification system, sonicator, sputter coater, critical point dryer, low speed bone saw, osmium plasma coater, and other equipment.