University of illinois Urbana-Champaign

Immersion Oils

Descriptions taken from Polysciences, Inc. LifeSciences Catalog 2000-2001

 

For Light Microscopy:

  • Type A: viscosity 150 centistokes - for short focus objectives
  • Type B: viscosity 1250 centistokes - for greater gap dimension
  • Type NVH: viscosity 21,000 centistokes - for horizontal, inverted, or inclined instruments

For Fluorescence Microscopy

  • Type DF: highest resolution, but slight green range background
  • Type FF: virtually no background fluorescence