Microscopy Suite Equipment Fees

Fees/rates for use of equipment in the Microscopy Suite determined through analysis by Government Costing, University of Illinois. In effect starting July 1, 2018; subject to change.


Leica SP8 UV/Visible Laser Confocal Microscope

Confocal microscopy permits one to optically section a fluorescent sample (such as a cell that has been stained with contrasting fluorescent dyes) with superior resolution by using a pinhole to reject light that originates outside of the chosen area. By collecting a series of such images through the depth of a sample, the user may assemble a highly accurate three-dimensional reconstruction of the entire sample.

Features:

  • Laser lines: 405, 458, 488, 514, 561 & 633 nm.
  • Four detectors: Three PMTs + one high-sensitivity GaAsP HyD detectors.
  • Resonant scanning (8kHz) allows 28 fps at 512*512 for fast live cell imaging.
  • Scan optics for high transmission from 400 nm to 1300 nm.
  • Optical scanfield rotation up to 200 degrees.
  • Three mirrors X2Y scanner for parallax-free and tiling imaging.
  • Motorized stage, mark & find, tiling confocal imaging.
  • LAS X control software.

Objectives:

  • 20x/0.75 IMM (oil, water, glycerol) CORR CS2, free Working distance 670 um
  • 40x/1.30 HC PL APO Oil CS2, free Working distance 240 um
  • 63x/1.40 HC PL APO Oil CS2, free working distance 140 um

Application Modules:

  • Fluorescence Recovery After Photobleaching (FRAP).
  • Fluorescence Resonance Energy Transfer (FRET).

 

 

For additional information about this piece of equipment, see the Calendars, Contacts, and Fees pages.

Primary Contacts
Manufacturer Leica Microsystems
Equipment Model TCS SP8
Location B420 B
Phone Numbers (217) 333-4387, (217) 265-5071, (217) 244-6270 (Room B420B)

Notes:

* The fee for use is added to our purchase cost for the specific precious metal (gold, gold-palladium, or platinum) expended during coating. Gold and gold-palladium are charged at $0.30/nm, and platinum is charged at $0.45/nm. The added fee is easy to determine for the dual-metal evaporator, for which total thicknesses are reported in nm. For the sputter coaters, at 65 mTorr and 40 mA, gold coats at 3 nm/10 seconds, gold-palladium coats at 1 nm/10 seconds, and platinum coats at 1 nm/10 seconds. So a standard 70-second coat of gold will cost an additional (21 x 0.30) $6.30, a 70-second coat of gold-palladium will cost (7 x 0.30) $2.10, and a 70-second coat of platinum will cost (7 x 0.45) $3.15.

**The Xradia NanoCT system requires staff setup of the sample before X-ray collection.

***The actual hourly cost for this instrument is $91.31. The Beckman Institute subsidizes the TEM at a rate of $41.31 per hour for Internal university users. Enterprise Works users are charged the full $91.31 per hour.