Fees
Microscopy Suite Equipment Fees
Fees/rates for use of equipment in the Microscopy Suite determined through analysis by Government Costing, University of Illinois. In effect starting August 1, 2021; subject to change.
Widefield FRET & TIRF Microscope
The Zeiss Axiovert 200M is configured to be capable of widefield Fluorescence Resonance Energy Transfer (FRET) microscopy and Total Internal Reflection Fluorescence (TIRF) microscopy. The microscope is equipped with a cooled Photometrics 512 Evolve EMCCD camera with high sensitivity for quantitative low-light fluorescence imaging.
FRET microscopy allows the determination of the approach between two molecules within several nanometers, a distance sufficiently close for molecular interactions to occur. For the FRET microscopy, the imaging and system control is realized by using the software MetaFluor. The filter sets installed in the microscope allow imaging a number of FRET pairs, such as ECFP-YPet.
TIRF microscopy can be used to observe the fluorescence of a single molecule or a thin region of a specimen, usually less than 200 nanometers thick. Currently three lasers (the wavelengths are 405 nm, 473 nm and 561 nm) are available for the TIRF imaging.
User Resources
For additional information about this piece of equipment, see the Calendars, Contacts, and Fees pages.
| Primary Contacts | |
|---|---|
| Secondary Contacts | |
| Manufacturer | Carl Zeiss, Inc. |
| Equipment Model | Axiovert 200M |
| Location | B0420A |
| Phone Numbers | (217) 333-4387, (217) 265-5071 |
Notes:
* The fee for use is added to our purchase cost for the specific precious metal (gold, gold-palladium, or platinum) expended during coating. Gold and gold-palladium are charged at $0.30/nm, and platinum is charged at $0.45/nm. The added fee is easy to determine for the dual-metal evaporator, for which total thicknesses are reported in nm. For the sputter coaters, at 65 mTorr and 40 mA, gold coats at 3 nm/10 seconds, gold-palladium coats at 1 nm/10 seconds, and platinum coats at 1 nm/10 seconds. So a standard 70-second coat of gold will cost an additional (21 x 0.30) $6.30, a 70-second coat of gold-palladium will cost (7 x 0.30) $2.10, and a 70-second coat of platinum will cost (7 x 0.45) $3.15.